Survival of avian influenza virus H6N2 in faecal samples of chickens experimentally infection
2013
Zarkov, I., Thrakia University, Stara Zagora (Bulgaria)
The persistence of infectivity of avian influenza virus AIV subtype H6N2 with initial titre 10 4.00 EID50 /100 μl was investigated in faeces of experimentally infected separately reared chickens under conditions of preserved humidity, ambient temperatures of 4 °C, 15 °C, 22 °C and рН 7.6. At the temperature of 15 °C and constant humidity, infectious virus was detected until the 6 day but not after a repeated inoculation on day 9. Virus titre reduction rate was slower until day 2 – by 10 0.5 EID50 /100 μl and faster between days 2 and 4 - by 10 1.17 EID50 /100 μl; then slowed down between days 4 and 6 – by 10 0.83 EID50 /100 μl and after the 6th day – the reduction was by 10 1.55 EID50 /100 μl until zero titre by the 9 day. At 4 °C and preserved humidity, the virus persisted until the 25 day with a titre of 10 0.5 EID50 /100 μl. Reduction of titres occurred with less than 10 0.1- 0.26 EID50 /100 μl by day 2 and 12, with more than 10 0.36 – 1.47 EID50 /100 μl between days 12 and 25 until zero titres by day 30. At 22 °C and preserved humidity the virus was detected up to the 2nd day with a titre of 10 1.0 EID50 /100 μl. Between days 1 and 2 titres declined by 10 1.5 EID50 /100 μl until zero titres by day 4.
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Este registro bibliográfico ha sido proporcionado por Institute of Agricultural Economics