Phenolic compounds content and morphogenesis in the liliac callus culture (Syringa vulgaris)

Aim of work was the determination of content total phenolics in the callus of leaf origin at cultivating in the media containing exogenic PC and their precursors, and establishment of connection between the total phenolics in the callus culture and processes of morphogeses in culture in vitro. Materials and methods of investigation. The object of investigation has been a stable callus culture of leaf origin of the lilac (Syringa vulgaris L.) 'M. Sholokhov' cultivar grown in the culture in the State Scientific Institution 'Central Botanical Gardens of NAS of Belarus'. Callus of leaf origin has been grown on Murashige and Skoog modified medium containing exogenic PC and their precursor substances: medium 1 - control medium not containing exogenic PC and their precursor substances; medium 2 - containing 6,1 mg /1 rutin; medium 3-6,1 mg/1 of quercetin; medi¬um 4 - 1,65 mg / 1 of phenylalanine; medium 5 - 6,1 mg / 1 of rutin + 1,65 mg /1 of phenylalanine; medium 6 - 6,1 mg /1 of quercetin + 1,65 mg /1 of phenylalanine. The period of cultivation is 60 days. 20th, 40th and 60th days corresponding to lag-, exponential and stationary phases of the lilac callus culture growth have been taken as analyzed intervals. 10 probes have been taken at each stage of investigation. Research results show that including in the medium of cultivation exogenic PC and their precursor substances allows to increase total phenolics in the callus culture of leaf origin and abridge cultivation time. Maximum total phenolics have been observed on the medium, containing 1,65 mg /1 of phenylalanine on the 20th day of cultivation. Formation of the embryogenic complexes takes place at low concentration of total phenolics in callus culture. Callus cultivation with the presence of exogenic PC and their precursor substances makes it possible to reduce the time of cultivation up to three weeks to form embryogenic structures.