Study on the antiendotoxin action of sinomenine, evodiamine and chuanxionggzine | 青藤碱、吴茱萸碱和川芎嗪抗细菌内毒素作用及其机制研究

Chino. 为探讨3种中药生物碱青藤碱、吴茱萸碱和川芎嗪对细菌内毒素（脂多糖,LPS）的治疗作用,ICR小鼠腹腔注射6 mg.kg-1的内毒素3 h后,根据分组情况分别再腹腔注射高（10 mg.kg-1）、中（5 mg.kg-1）、低（1 mg.kg-1）3个剂量的青藤碱、吴茱萸碱和川芎嗪,并设立LPS攻毒组、空白对照组和药物对照组。记录LPS攻毒后48 h内各组小鼠临床表现,测定体温、体质量及器官指数,检测血清中LPS和炎症因子的含量。结果显示：青藤碱和川芎嗪能显著抑制体温升高,吴茱萸碱能显著抑制体质量下降和血清中LPS水平升高,青藤碱能显著降低血清中炎症因子含量增加,吴茱萸碱和青藤碱能显著改善小肠肿大等病变。结论：青藤碱、吴茱萸碱和川芎嗪3种中药生物碱具有明显的抗细菌内毒素作用。

Inglés. To investigate the antiendotoxin action of sinomenine,evodiamine and chuanxionggzine,mice in treatment groups were challenged with 6 mg・kg-1 lipopoly saccharide（LPS）by peritoneal injection,and then treated with sinomenine,evodiamine and chuanxionggzine at three concentrations（1,5 and 10 mg・kg-1）.The mice in LPS group were challenged with 6 mg・kg-1 LPS by peritoneal injection.The mice in drug groups were treated with 10 mg・kg-1 sinomenine,evodiamine and chuanxionggzine by peritoneal injection.The mice in blank group were treated with physiological saline.The determined items and results were as follows：compared with LPS control group,the body temperature significantly decreased in the high-dose group of sinomenine and the middle-and high-dose groups of chuanxionggzine;the body weight significantly increased in the high-dose group of evodiamine;the LPS contents significantly decreased in the middle-and high-dose groups of evodiamine;the tumor necrosis factor alfa（TNF-α） contents significantly decreased in the middle-and high-dose groups of sinomenine;the interleukin 1 beta（IL-1β）contents significantly decreased in the middle-and high-dose groups of sinomenine;the index of intestinal significantly decreased in the high-dose groups of sinomenine and evodiamine.It suggested that sinomenine,evodiamine and chuanxionggzine had significant antiendotoxin effect,thus to providing a theoretical basis for clinical application of alkaloid in controlling the diseases caused by LPS.