Detection and characterization of Leptospira spp. isolated from aborted bovine clinical samples
2012
Momtaz, H., Islamic Azad Univ., Shahrekord (Iran Islamic Republic). Dept. of Microbiology | Moshkelani, S., Islamic Azad Univ., Shahrekord (Iran Islamic Republic). Young Researchers Club
The aim of the present study was to detect and characterize Leptospira as the causative agent of abortion in cattle using a PCR-RFLP in Chaharmahal va Bakhtiari and Isfahan provinces, Iran. A total of 220 bovine aborted foetuses and 120 vaginal discharges were collected from 64 commercial dairy herds. After isolation of 60 Leptospira spp. from samples, RFLP analysis was carried out with HindIII and HaeIII restriction enzymes. In a total of 340 specimens, 46 and 14 were identified positive for Leptospira spp. from aborted bovine foetuses and vaginal discharges, respectively. The present results also suggest that L. interrogans serovar hardjo has the highest prevalence in the region under study and L. hardjo is a major pathogen causing bovine abortion in Chaharmahal va Bakhtiari and Isfahan provinces of Iran.
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