Isolation, cloning and sequence analysis of the lactate dehydrogenase gene from Theileria annulata may lead to design of new antitheilerial drugs
2012
Erdemir, A., Yildiz Technical Univ., Istanbul (Turkey). Faculty of Chemical and Metallurgical Engineering | Aktas, M., Firat Univ., Elazig (Turkey). Dept. of Parasitology | Dumanli, N., Firat Univ., Elazig (Turkey). Dept. of Parasitology | Turgut-Balik, D., Yildiz Technical Univ., Istanbul (Turkey). Faculty of Chemical and Metallurgical Engineering
The isolation, cloning and analysis of lactate dehydrogenase from T. annulata was the goal of the present study with the ultimate aim of designing new antiparasitic drugs. Blood samples were taken from a four year-old Brown Swiss cow showing signs of acute tropical theileriosis and genomic DNA was extracted following the confirmation of the clinical diagnosis. The lactate dehydrogenase sequence was isolated from a Theileria species for the first time. After the extraction from genomic DNA by PCR the sequence was cloned into the vector pGEM-T. Sequencing of the whole gene from both directions indicated that the open reading frame was interrupted by two introns. Several single nucleotide exchanges, deletions and insertions were also observed in the T. annulata lactate dehydrogenase sequence compared to the host. The most remarkable difference between the parasite and host enzyme is a five residue insertion in the active site loop region that might be an attractive target for inhibitors of the enzyme.
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