Trials for propagation and adaptation of egg drop sundrome virus EDS-76 on chicken embryo fibroblast cell culture and SPF embryonated chicken eggs
2010
Ibrahim, N.M. | Abo-Zaid, A.Z.A.
Duck embryo adapted strain of EDS-76 (EID50 10. /ml) was propagated and adapted on chicken embryo fibroblast. Thirty serial passages were prepared and titrated. After every 5 passages, the virus was inoculated into chickens 21 days old and observed for 21 days post-infection, then, challenged with a virulent EDS-76 virus. The 24th passage induced complete loss of virus pathogenicity and gave 100% protection, and used for the preparation of a live attenuated and inactivated oil emulsion chicken embryo fibroblast (CEF) cell culture (EDSV) vaccines. The evaluation of the prepared vaccines was carried out for sterility, safety and potency. The potency test was performed by measuring the cellular and humoral immune response, as well as, the protection percentage after challenge. The efficiency of the prepared vaccine was estimated up to six months. Passage number 15 and passage number 30 of EDS virus adapted on CEF cell culture were inoculated on SPF embryonated chicken eggs propagation and titration for successive seven passages. Haemagglutination (HA) was estimated to collected materials (allantoic fluids-chorioaliantoic membraneembryonic extract) by rapid haemagglutinating EDS virus and slow quantitative Haemagglutination reaction.
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