Analysis of genetic structure of Iranian bactrian camel, using microsatellite markers
1998
Afraz , F | Banabazi, M | Asadi, N | Razavi , K
In this study, we have investigated genetic variation within Iranian two-humped camel populations, using sixteen microsatellite markers; CMS9, CMS15, CVRL01, CVRL05, CVRL07, LCA33, LCA56, LCA63, LCA66, LCA77, VOLP03, VOLP10, VOLP67, YWLL08, YWLL38 and YWLL59 from new and old world camelids. DNA extract was done from the blood samples of 110 individual with optimized and modified salting-out method. The polymerase chain reactions (PCR) were successfully done with all primers and then amplification products were resolved in 8% (undenaturing) PAGE and stained with silver nitrate. Hence, we observed 56 alleles and except for CMS15 and LCA77, all primers were found polymorphic. Also, polymorphism ratio (P) for nine microsatellite primers in this population calculated 87.50 percent. Allelic and polymorphic information content (PIC) averagesfor all loci were 3.50 and 0.427, respectivly. Significant deviations from Hardy-Weinberg equilibrium (HWE) occurred for all loci (P0.005). The average expected heterozygosity with monomorph loci calculated 0.476 and ranged from 0.165 to 0.771. The phylogeny denderograms based on the DA distances were drown using unweighted pair-group method by an arithmetic average (UPGMA) for inside population, UPGMA dendrogram cleave this population the two main groups and for each one, cleavage to multiple secondary groups. On the basis of these findings of this research, 1st we can conclude that the Iranian two-humped camel population have acceptable genetic diversity and could be preserved from extinction by optimal breeding and management programs and 2nd microsatellite markers could be an useful tool for screening of biodiversity in domestic animals.
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