Free Radical Scavenging Activity and Protective Effect against H₂O₂-Induced Stress in Neuronal Cells of Enzymatic Extracts from Sarcodon aspratus
2011
Lee, S.J., Konkuk University, Chungju, Republic of Korea | Kim, E.K., Konkuk University, Chungju, Republic of Korea | Oh, H.J., Konkuk University, Chungju, Republic of Korea | Kwon, H.J., Konkuk University, Chungju, Republic of Korea | Hwang, J.W., Konkuk University, Chungju, Republic of Korea | Moon, S.H., Korea Nokyong Research Center, Konkuk University, Chungju, Republic of Korea | Jeon, B.T., Korea Nokyong Research Center, Konkuk University, Chungju, Republic of Korea | Park, P.J., Konkuk University, Chungju, Republic of Korea | Lim, B.O., Konkuk University, Chungju, Republic of Korea
The antioxidative activity of various enzymatic extracts from Sarcodon aspratus (S. aspratus) was evaluated by measuring 1,1-diphenyl-2-picrylhydrazyl (DPPH), and alkyl radical scavenging activity using an electron spin resonance (ESR) spectrometer. For this study, the S. aspratus were enzymatically hydrolyzed by seven carbohydrases (Viscozyme, Celluclast, Dextrozyme, AMG, Promozyme, Maltogenase, and Termamyl) and eight proteases (α-chymotrypsin, Alcalase, Flavourzyme, Neutrase, papain, pepsin, Protamax, and trypsin). The DPPH radical scavenging activities of Viscozyme and pepsin extracts were the highest, and the half maximal inhibitory concentration (IC∧50) values were 0.896 and 0.734mg/mL, respectively. The Celluclast and trypsin extracts showed the highest scavenging activities on alkyl radical, and their IC∧50 values were 0.278 and 0.575mg/mL, respectively. The Celluclast extracts was decreased cell apoptosis in PC-12 cells against H₂O₂-induced oxidative damage. The findings of the present study suggest that enzymatic extracts of S. aspratus exhibit antioxidative activity against oxidative stress on PC-12 cells.
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