Effect of Thymidine on In Vitro Maturation of Immature Porcine Follicular Oocytes
2012
Min, S.H., Daegu University, Gyeongsan, Republic of Korea | Park, H.D., Daegu University, Gyeongsan, Republic of Korea
The objective of this study was to examine the effect of thymidine treatment during in vitro maturation (IVM) of porcine follicular oocytes on blastocyst development. Porcine oocytes were treated with thymidine (10 mM, 20 mM and 30 mM) for 2 or 6 hr in the preiods of IVM Ⅰ and/or Ⅱ. The survival rates of the blastocysts in the 6 hr treatment groups of 10 mM and 20 mM during IVM Ⅰ period were significantly higher than those of control group (p less than 0.05). However, the survival rate of the blastocysts in the 2 hr treatment group of 20 mM during IVM Ⅱ period was significantly higher than control group (p less than 0.05). Furthermore, the survival rate of the blastocysts in the 6 hr treatment group of 30 mM during IVM Ⅱ period was significantly lower than control group (p less than 0.05). Consistent with the previous result, blastocyst development of both IVM Ⅰ and Ⅱ treatment group was also showed as similar pattern. Total and apoptotic cell numbers of blastocysts derived from thymidine treated porcine oocytes were examined by using Tunel assay. The results showed that there was no significant differences in total cell number of blastocysts between thymidine treated and untreated groups. However, apoptosis-positive cells in the thymidine treated group (6 hr IVM Ⅰ) were significantly lower than those of other groups (p less than 0.05). Taken together, these results indicate that high quality oocytes were selected by DNA synthesis mechanism according to high concentration thymidine treatment during porcine oocyte maturation. Therefore, we concluded that presumptive selected oocytes by thymidine treatment during maturation periods improved the further embryo development and embryonic quality of IVF embryos by decreasing the incidence of apoptosis in preimplantation porcine embryos.
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