Isolation and identification of Verticillium dahliae causing wilt on potato in Pakistan
2012
Ashraf, A. (PMAS arid Agriculture Univ., Rawalpindi (Pakistan). Dept. of Plant Pathology) | Rauf, A. (National Agricultural Research Centre, Islamabad (Pakistan)) | Abbas, M.F. (National Agricultural Research Centre, Islamabad (Pakistan)) | Rehman, R.
During the year 2010-11 and 2011-12, disease incidence of Verticillium dahliae in Rawalpindi (Taxila and Adyala) and Islamabad (National Agricultural Research Centre) was investigated in farmer's potato fields. During the year 2010-11, maximum mean incidence was 24, 15 and 9% in Taxila, Adyala and NARC region respectively. Mean disease incidence increase 1% and 8% in Islamabad and Adyala while it was reduced by 3% in Taxila region respectively, during the second year of investigation. Over all mean disease incidence was increased by 2% comparison to disease incidence during second year. Maximum size of microsclerotia was recorded 63.9 pm in NARC while Taxila has smallest size of microsclerotia 35.7pm. Colony was dark brown to black and conidiophores were phialides, erect and septate. Ovate-oblong and elliptical conidia were singly associated to end of terminal branches before falling away. Taxila and Adyala isolate showed virulent behavior and Islamabad isolates were found the least virulent on Cardinal and Coroda. Most suitable temperature, pH and culture media were 26 degree C, 7 and verticillim specific media for the growth of V. dahliae respectively.
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