Screening of cadmium tolerance in sugarcane using antioxidative enzymes as a selection criteria
2013
Raza, S.H. (Government Coll. Univ., Faisalabad (Pakistan). Dept. of Botany) | Shafiq, F. (Government Coll. Univ., Faisalabad (Pakistan). Dept. of Botany) | Tahir, M. (Government Coll. Univ., Faisalabad (Pakistan). Dept. of Botany)
Impact of cadmium stress on sugarcane (Saccharum officinarum L.) callus was investigated to screen the tolerant and sensitive varieties. Fresh, young and disease free leaves of four sugarcane varieties (HSF-240, HSF-242, CPF-246 and CPF-247) were used as explants to induce callus on MS medium. Callus (25-Day old) was subjected to various cadmium chloride levels (control, 0.1, 0.5 and 1 mM) and data for growth was recorded after 7 and 15 days however enzyme activities were recorded after 10 days, respectively. Results clearly highlighted the detrimental effects of cadmium stress on all the four varieties. Morphologically all the varieties showed red patches, and a significant alteration in fresh and dry weights were recorded after 7th and 15th days of cadmium exposure. HSF-240 showed 11% and 16% increase in fresh and dry weight after 7th and 15th days respectively as compared to other varieties. Furthermore, total protein, CAT and POD activities were positively triggered under Cd stress especially in HSF-240. Na+ and K+ concentration varied under cadmium treatment in a way that HSF 240 had 11% more values of K+ than HSF-242, while HSF-242 showed 11% higher values than other two varieties (CPF-246 and CPF-247). Based on the findings, HSF-240 was Cd-tolerant while HSF-242, CPF-246, and CPF-247 were found susceptible to cadmium.
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