Scutellum-derived callus-based efficient and reproducible regeneration system for elite varieties of indica rice in Pakistan
2013
Joyia, F.A. (National Inst. for Biotechnology and Genetic Engineering, Faisalabad (Pakistan)) | Khan, M.S. (University of Agriculture, Faisalabad (Pakistan). Centre of Agricultural Biochemistry and Biotechnology)
An expeditious regeneration system was established using scutellum-derived calli from mature seed of six Pakistani rice varieties namely, (Super Basmati, Basmati 385, Basmati 198, Pak Basmati, Basmati 2000 and Basmati 370) using modified MS medium containing various 2,4-D levels ranging from 1.0 to 5.0 mg/L. For regeneration, the embryogenic callus was sub-cultured on MS medium supplemented with 3.0 mg/L of kinetin and 1.0 mg/L of NAA. Increase in 2,4-D concentration enhanced the callus induction and proliferation but, had negative influence on the regeneration potential of calli. Experiments were performed to optimize the responsive age of the cells to regeneration, which was a prime character for efficient regeneration. Furthermore, effects of various gelling agents and carbon sources were also studied. Here, we report an efficient and reproducible regeneration system where responsive age of calli was minimized to 3-5 days with maximum number of shoots produced within a period of three weeks. The regenerates were transferred to soil for acclimatization. Though such research findings have already been published but these results are unique as far as responsive age of cells to regeneration and total time to produce shoots from cells are concerned.
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