Carbohydrate metabolism and antioxidant defense during diapause development in larvae of oriental fruit moth (Grapholita molesta) at low temperature
2013
He, C. (Northwest A and F Univ., Shaanxi (China). Coll. of Plant Protection) | Quan-Ke, M. (Sanmenxia Polytechnic, Henan (China). Dept. of Biology and Chemical Engineering) | Xiang-Bin, Y. (Northwest A and F Univ., Shaanxi (China). Coll. of Plant Protection) | Hua, L.
Oriental fruit moth, Grapholita molesta (Lepidoptera: Tortricidae), is a major pest of tree fruits worldwide, overwinters in mature larva diapause. However, little is known about the physiological explanation for diapause development in this pest. In this study, we analyzed the dynamic metabolic processes of carbohydrate, protein and antioxidant enzymes in diapausing larvae, which were maintained at low temperatures of 4 and 10 degree C under laboratory conditions, and 24 degree C as control for up to 4 months. After 100 days of maintenance, diapause could be terminated at low temperatures, as indicated by the pupation rate, the larvae were more conducive to complete diapause development at 10 degree C compared to 4 degree C. During diapause, total sugar and glycogen content decreased and trehalose content increased at low temperatures; glycerol content significantly increased at 4 degree C, but not at 10 degree C; protein content did not significantly changed at low temperature, but slightly decreased during the middle phase of diapause. Additionally, the activities of SOD and CAT increased and POD activities decreased during the middle phase of diapause at low temperatures. Our results indicate that temperature and diapause intensity significantly affected the carbohydrate content and antioxidant enzymes activities of the diapausing larvae, low temperature plays a predominant role in diapause termination, and antioxidant enzymes have a similar role in the regulation of energy metabolism as carbohydrate.
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