Effect on Insecticide Susceptibility of Lissorhoptrus oryzophilus Fed on Bacillus thuringiensis (Bt)-Transgenic Rice Variety
2012
Oh, S.D., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea | Lee, K.J., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea | Sohn, S.I., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea | Kwon, Y.J., Kyungpook National University, Daegu, Republic of Korea | Kim, J.S., Kyungpook National University, Daegu, Republic of Korea | Lee, J.Y., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea | Park, B.S., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea | Kweon, S.J., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea | Suh, S.C., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea | Ryu, T.H., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea | Park, J.S., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea | Ahn, B.O., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea | Cho, H.S., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea | Suh, S.J., Kyungpook National University, Daegu, Republic of Korea
Insect-resistant transgenic rice, Bt rice was developed by inserting the mCry1Ac1, a modified gene from the soil bacterium Bacillus thuringiensis (Bt). For the biosafety assessment of this GM rice, we studied the effect on insecticide susceptibility of Rice Water Weevil (Lissorhoptrus oryzophilus) which is a nontarget insect pest and commonly used as a model organism in ecotoxicological studies. The Bt rice was confirmed to have the insertion of T-DNA and the protein expression by the polymerase chain reaction (PCR) and the ELISA analysis, As for the cumulative mortalities of Rice Water Weevil on insecticide, Clothianidin in Bt and non-GM counterpart, Nakdong rices were similar. The 72h-LC∧50 values of Bt and non-GM rices showed 0.245 ml/L and 0.257 ml/L respectively, As the 72h-LC∧50 vlaue of Bt rice was included within the 95% confidence limit of that of Nakdong rice slightly higher, the two varieties of the 72h-LC∧50 values were not significant, Thus, we found out there was a strong possibility that Bt rice didn't affect the insecticide susceptibilities of nontarget insects.
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