Comparison of methods for relative quantification of gene expression using real-time PCR | Primerjava metod za relativno kvantifikacijo genskega izrazanja s PCR v realnem casu
2012
Bolha, L., Ljubljana Univ. (Slovenia). Biotechnical Fac., Dept. of Animal Science | Dusanic, D. | Narat, M. | Oven, I.
Quantitative real-time PCR (qPCR) has become a widely used tool for quantifying gene expression. Several methods for relative quantification have been developed, enabling rapid and reliable detection and quantification of specific nucleic acids. These methods, based on qPCR include: the standard curve method, the efficiency calibrated method and the 2−ΔΔCq method. Here we analyzed if these three m
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