Antiviral activities of Boletus edulis, Pleurotus ostreatus and Lentinus edodes extracts and polysaccharide fractions against Herpes simplex virus type 1.
2012
Santoyo, S., Universidad Autónoma de Madrid, Madrid (Spain) | Ramírez-Anguiano, A. C., Universidad Autónoma de Madrid, Madrid (Spain) | Aldars-García, L., Universidad Autónoma de Madrid, Madrid (Spain) | Reglero, G., Universidad Autónoma de Madrid, Madrid (Spain) | Soler-Rivas, C., Universidad Autónoma de Madrid, Madrid (Spain)
Antiviral properties of water and methanol extracts from Lentinus edodes, Boletus edulis and Pleurotus ostreatus were evaluated against herpes simplex virus type 1 (HSV-1). Pre-treatment of Vero cells with 75 mg per ml water extracts before virus addition, inhibited 60% of the virus infection and its addition during the virus adsorption period inhibited it up to 80%. Moreover, the mushrooms water extracts were able to significantly inhibit the in vitro virus replication, showing the concentration of a substance required to reduce plaque number in Vero cells by 50% (IC50) values from 26.69 mg per ml to 35.12 mg per ml. Methanol extracts exhibited a lower antiviral activity in all cases. In order to identify the type of compounds responsible for the antiviral activity, the low molecular weight (LMW) and high molecular weight (HMW) fractions were evaluated. The activity was found to correlate with the beta-glucans present in the polysaccharide fractions, which showed higher antiviral activity than the complete water extracts except for B. edulis where other compounds (probably chitin-binding lectins) might have contributed to the observed activity.
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