Using heavy-ion mutagenesis technology to select cellulose enzyme vitality of mutants of Aspergillium niger | 重离子辐照诱变黑曲霉产纤维素酶菌株的筛选
2012
Tang Jiahui, Gansu Agricultural University, Lanzhou (China), College of Food Science and Engineering | Yang Fumin, Gansu Agricultural University, Lanzhou (China), College of Food Science and Engineering | Wang Shuyang, Chinese Academy of Sciences, Lanzhou (China), Institute of Modern Physics
Chino. 为提高黑曲霉（=AS3.316）产纤维素酶的活力，用20、40、60、80、100、120、140Gy剂量的重离子束对其进行辐照诱变，并通过初筛、复筛筛选出了纤维素酶活力提高的菌株。结果表明：通过重离子束辐照共筛选出了5株突变体T2-1、T3-1、T5-1、T6-3、T6-4，其中突变体T6-4的纤维素酶活力最高，滤纸酶、纤维素内切酶、纤维素外切酶、β-葡萄糖苷酶活力分别为：61.3、116.2、29.9、35.9U/mL，与初始菌株相比纤维素酶活力分别提高了3.0、3.78、2.76和2.52倍；其余突变体纤维素酶活力大小依次为T6-3T5-1T3-1T2-1；120Gy辐照条件下，诱变效果最为显著；经9代传代稳定性试验，5株突变体在传代过程中虽有不同程度的变化，但总体表现相对稳定。
Mostrar más [+] Menos [-]Inglés. In order to improve the cellulose ion beam at 20, 40, 60, 80, 100, 120Gy and 140 enzyme vitality of Aspergillus niger (=AS3.316), heavy Gy doses was used for inducing mutation. Higher cellulose enzyme vitality strains were screened through the primary screening and secondary screening. The result showed that 5 mutants T2-1, T3-1, T5-1, T6-3, T6-4 were selected, and T6-4 had the highest cellulose enzyme activity. The activity of filter paper cellulose enzyme, endo-glucanase, exo-glucanase and 13-glucosidase of T6-4 was 61.3, 116.2, 29.9 U/mL and 35.9 U/mL respectively. Compared with the original A. niger (=AS3.316), the cellulose enzyme activity was increased by 3.5, 3.78, 2.76 and 2.52 times in turn. The activity of cellulose enzyme of the rest mutants sorted from strong to the weak were T6-3T5-1T3-1T2-1. The dose at 120 Gy showed the best mutagenesis effect. Mutants had different degree of changes in the genetic stability, but overall, the performance showed relatively stable.
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