«BioMolChem», a tool to assess the defense status of grapevines after stimulations or not: from laboratory to the field
2013
Corio-Costet , Marie-France (INRA , Villenave D'Ornon (France). UMR 1065 Santé et Agroécologie du Vignoble) | Cluzet , Stéphanie (Institut National de la Recherche Agronomique, Villenave d'Ornon(France). UMR 1065 SAVE : Santé et Agroécologie du Vignoble) | Lambert , Carole (Institut National de la Recherche Agronomique, Villenave D'Ornon(France). UMR 1065 SAVE : Santé et Agroécologie du Vignoble) | Dufour , Marie-Cecile (INRA , Villenave D'Ornon (France). UMR 1065 Santé et Agroécologie du Vignoble)
Stimulating plant defenses is a promising alternative method for limiting pesticide use in agriculture. To assess the defense status of the grapevine we have developed a triple approach called "BioMolChem".- Biological tests measure the efficacy of grapevine defenses against two major biotrophic pathogens (Erysiphe necator, Plasmopara viticola).- Molecular assays by q-RT-PCR show the expression patterns (over-expression or repression) of 24 genes involved in grapevine defenses, and they can then be correlated or not with the level of protection,- BioChemical analyses of phenylpropanoides by HPLC are used to quantify and identify molecules of interest, and correlate them with specific gene expression (stilbene biosynthesis) and the acquired protection.This tool was tested on leaves after stimulation by different elicitors (acibenzolar-S-methyl, phosphonates), on grapevine: Cabernet Sauvignon cv., resistant genotypes to powdery and downy mildew, and in the vineyard. We obtained correlations between the expression of various genes and with the level of protection. Similarly, we found correlations between the presence of known and unknown polyphenols and the level of protection. Resveratrol, a well-known phytoalexin of the grapevine, is a good marker of defense status but not of protection. Therefore, we now have an valuable tool for understanding the defense and protection status of the grapevine in laboratory and field experiments.
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