The use of ACC deaminase producing pseudomonads for increasing bioremediation efficiency in polluted soils

Soil pollution to the oil hydrocarbon products is one of the soil pollution kinds that is occurred widespread in the equipment surroundings related to the exploration and refine of the oil. Also, the soil pollution is observed regionally in the transport pathways of these products in the southern oil provinces and other provinces of the country. Phytoremediation is one of the preferred methods that is effective and less expensive, which is used for removing this kind of polluttion from these oil polluteded soils. This method efficiency is generally generated from the growth amount of the mentioned plant in the polluted conditions. In these conditions, because of the ethylene accumulation in the plant tissues, plant growth decreases. Therefore, with the probability of ethylene production decrease in the plant, we could prepare better growth conditions, and improve eficiency method of bioremediation. Keeping in mind, plant inoculation with the bacteria that are capable of producing ACC deamimase, it could decrease the concentration of the precursor of ethylene production in the plant, meanning ACC (1- Amino cyclo carbon 1- Carboxylic acid), which will decrease the ethylene formation in the plant, or it will delay the process. For the confirmation or rejection of this hypothesis (H0), the multi strain effect of these pseudomonads, that are capable of producing the above enzyme, for investigating the efficiency amount of bioremediation in the plants such as; corn, barely, and cannola of the oil polluted soils. Each one of these experiments, which is carried out individually in the greenhouse; in a completely radomized design including five treatments with inoculating bacteria; Pseudomonas putida strain 4، Pseudomonas putida strain 108، Pseudomonas fluorescens strain169، Pseudomonas fluorescens strain12 ، Pseudomonas fluorescens strain9, and the control treatment without the inoculation, which were replicated four times. In all the carried out indiviual experiments, the effect of the studied strains on the plant growth indexes of the corn, barely and canola was significan at the α =1% level. In the corn and barely case, the experiment of the P12 strain had the most effect on the plant growth indexes of these plants in the oil polluted soils. In the canola case, the most effective strain, was the R169 strain, but there was no significance with the other strains and Including P12 strain. Over all, the P12 strain could be introduced for increasing plant growth in the oil polluted soil conditions. We also concluded that, in the case of removing aromatic hydrocarbons with multi rings (PAHs) and also in no plant conditions the P12 and P9 strains, in the corn and canola growth conditions the R169 and R108 strains, and in the barely case the P9 strain had the most effect.