Preparation of inactivated vaccine to induce resistance against White Spot Syndrome Virus by nuclear & non nuclear techniques

The inactivated of white spot syndrome virus (WSSV) by Gama and Electron radiation and chemical material such as Formalin with antigen property have been done during last year. At the first time the stock of virus prepared and purred it. Six hundred of shrimp Fenneropenaus indicus with average weight 7 to 12 gram collected from shrimp research station in Heleh area and transported to Shrimp Research Institute in Boushehr province. Vibrio parahemolyticus obtained from Veterinary University of Tehran and then lyophilized it. The bacterial V. parahemolyticus inactivated with Gamma, electron and formalin and then injected to shrimp for activated the immune system. The source of viruses was identified through PCR, TEM and histopathology methods from the shrimp infected in 2009 occurrence of boushehr province. The virus injected to crayfish and collected the hemlymph for prepared the stock of WSSV virus. The virus was lyophilized and then exposed to gamma and electron radiation and formalin with optimal dose for inactivated the virus. The shrimp divided to 25 groups and each group consist of six shrimp and the vaccine exposed to shrimp with injected and bathroom methods and the documented the result after 15 days. In vivo virus titration was performed in Penaeus indicus. Inactivation of WSSV was carried out by a gamma cell instrument Nordian, model 220 with dose rate: 4.8 Gy/sec and activity: 20469 Ci. The LD50 of live virus stock was calculated 10 5.4 / ml and the optimum dose of gamma radiation beam to inactivate WSSV was obtained 14-15 kGy. The LD50 of live virus stock was calculated 10 5.4 / ml and the optimum dose of electron beam to inactivate WSSV was obtained 12-13 kGy and and the virus inactivated by formalin 0.5% V/V during 10 minutes.The LD50 of live virus stock was calculated by Karber method 10 3.29 /ml and 10 5.35 /ml, respectively. The V. parahemolyticus was inactivated with 8 KG. The result showed all vaccine during 2 hours have a good effect to shrimp viruses with the102.4 LD50 / 50 l titre. The result showed if the Gamma and electron vaccine increase to feed of shrimp the effect of vaccine is better than when used without feed. The end of experiment our study showed that the Gamma vaccine has the better effect to control WSSV during 2 hours with comparing the others.