Ketamine-Xyline increases streptozotocin toxicity through improvement process of lipid peroxidation

Peroxidation of unsaturated fatty acids has been hypothesized to be a causal factor in many degenerative diseases including aging, heart diseases, cataract and diabetes mellitus. According to Freeman and Crapo (1982), anaesthesia agent is one of source off free radicals which having potency to cause various physiological complication through lipid peroxidation mechanism. Adji (1997) has also proven that ketamine-xylazine anaesthesia increased lipid peroxidation in rat after fed atherogenic diet. The purpose of the research was to see the effect of ketamine-xylazine in diabetic animals. Thirty adult female Sprague Dawley rats, 3 months of age, 250 grams of BW were used in this research. Rats were adapted for I week, fed on a standard diet and water ad libitum. Rats were then divided in to 6 groups, 5 for each. Group I was used as control animals, they were not injected with streptozotocin nor ketamine-xylazine; Group II was injected by 40 mg/kg BW of streptozotoein intraperitoneally without ketamine-xylazine injection. Group III was injected with 50 mg/kg BW of streptozotoein, intraperitoneally without ketamine-xylazine injection. Group IV was injected with 90mg / kg BW of ketamine combined with 10 mg/kg BW of xylazine without streptozotocin injection. Group V was injected with 40 mg/kg BW of streptozotocin intra peritoneally and 90 mg/kg BW of ketamine combined with 10 mg/kg BW of xylazine, intramuscularly. Group VI was injected with 50 mg/kg BW of streptozotocin intraperitoneally, and 90 mg/kg BW of ketamine combined with 10 mg/kg BW of xylazine , intramuscularly. The ketamine-xylazine injections were done 3 days after streptozotocin treatment. Thirty minutes after ketamine-xylazine injection, blood samples were collected from orbital vein in order to measure glucose, total cholesterol and thiobarbituric acid reactive substance (TBARs) concentration. Statistical analysis using multifactorial analysis of variance for all collected datas showed that there were significant differences between glucose, total cholesterol and TBARs plasma concentration among the treatments (P < 0.05). Based on the present study it can be concluded that ketamine-xylazine HCI increased streptozotoein toxicity through lipid peroxidation.