Molecular and morphological comparisons between for east and mediterranean Anisakis pegrefii Campana-Rouget et Biocca, 1955
2011
Quiazon, K.M.A. | Yoshinaga, T. | Ogawa, K., Tokyo Univ., Yayoi 1-1-1, Bunkyo-ku, Tokyo 113-8657(Japan). Dept. of Aquatic Biosciences | Mattiucci, S., Rome 'La Sapienza' Univ., Piazzale Aldo Moro, 5, 1-00185 Rome (Italy). Inst. of Parasitology | Santos, M.D., Tokyo Univ. of Marine Science and Technology, Konan 4-5-7 Minato-Ku, Tokyo 108-8477 (Japan). Lab. of Genome Science
Anisakis pegreffii Campana-Rouget, et Biocca, 1955 has been widely reported, not only in the Mediterranean and Atlantic but also in the Far East region. Recently, two base differences was detected in the sequence of 5.8S rDNA between A.pegreffii from the Mediterranean and that from the Far East region, and the latter was tentatively designated as Anisakis pegreffii JP based on the difference. In this study, A.pegreffii from the Mediterranean and from the Far East were morphologically and molecularly compared. Morphologically, little difference was detected, including the ventriculus length, among the specimens. In PCR-RFLP [polymerase chain reaction-restriction fragment length polymorphism] and DNA sequences of ITS rDNA, identical results were obtained from all the present and previously reported specimens of A. pegreffii except that the two-base differences was detected in only one sequence reported from the Mediterranean (GenBank Acc. No. AY826720) in which an unclear base was sandwiched between the different base positions, suggesting possible error in sequencing. In mtDNA cox2, no geographical difference was also detected. These results indicate that A.pegreffii JP is identical morphologically and molecularly with other A.pegreffii.
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