Genetic characterization of different tomato varieties on the basis of atpB gene promoter
2013
Malik, S. (Quaid-i-Azam Univ., Islamabad (Pakistan). Dept. of Plant Sciences) | Mahmood, T. (Quaid-i-Azam Univ., Islamabad (Pakistan). Dept. of Plant Sciences)
Several chloroplast genes have been studied so far, among them is single copy large atpB gene, which encodes beta-subunit of ATP synthase. ATP synthase is present in membranes of mitochondria, chloroplast and prokaryotes having primary function of ATP synthesis. With the aim to gain discernment into the functions and regulation, promoter of atpB gene (Tilde 1000bp) from five selected tomato varieties (Moneymaker, Nagina, Punjab chuara, Riogrande and Roma) was amplified, sequenced and analyzed. The sequence analysis was done using BLASTn, MEGA5 and PLACE/Web Signal Scan. Functional significance of each regulatory element was also studied by using already available data. The atpB gene promoter sequence data from the varieties has been submitted to GenBank. Succinctly it can be recognized that atpB gene promoter sequences from five tomato varieties have shown variation and genetic diversity and ensure a wide range of common and unique cis-regulatory elements having variance in their copy number and location. These elements are functionally known to be significant in gene regulation and metabolism.
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