Novel OYE interactors define a cytoskeletal module in yeast
Ben Ghorbal, A.
Aging is the progressive decline in the ability of an organism to resist stress, damage, and disease. In yeast, the accumulation of oxygen-containing free radicals in the cell results in oxidative stress conditions that can affect the actin cytoskeleton, as an early target, and cause apoptosis and cellular senescence. The Old Yellow Enzyme 2 (OYE2) member of FMN-oxidoreductases has been characterized to regulate oxidation between C285 and C374 residues of the actin molecule in Saccharomyces cerevisiae. Additionally, it has been implicated in the suppression of Bax lethality in yeast, in lowering the endogenous reactive oxygen species (ROS), in increasing resistance to H2O2-induced programmed cell death (PCD) and significantly lowering ROS levels generated by organic prooxidants and promoting cell death upon oxidative stress via oye2p-oye3p heterodimer form. In the presence of such diverse functions, the identification of OYE2 interacting molecules can help in elucidating the full extent of its roles in the cell as well and could enhance our understanding of the fundamental processes' of aging and apoptosis. In this study, a yeast two hybrid system, which is a powerful screening strategy, was employed to search for interactors of the OYE2 yeast protein implicated in programmed cell death. In this screen we identified six specific interactors: PAN1, ARPC3, SKG3, APE2, AFT2 and TSA2 strengthening the initial evidence for participation of OYE2 in endocytosis, polarized cell growth, dynamics of actin cytoskeleton, and cell response to stress conditions.
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