Inhibitive activity of Kluyveromyces marxianus on Penicillium digitatum Sacc. | 1株拮抗酵母菌对柑橘绿霉病菌的抑制作用
2013
Luo Jianjun, South China Agricultural University, Guangzhou (China), Key Laboratory of Natural Pesticide and Chemical Biology | Geng Peng, South China Agricultural University, Guangzhou (China), Key Laboratory of Natural Pesticide and Chemical Biology | Hu Meiying, South China Agricultural University, Guangzhou (China), Key Laboratory of Natural Pesticide and Chemical Biology
Chino. 以柑橘绿霉病菌（Penicillium digitatum Sacc．）为供试材料，研究拮抗酵母菌（Kluyveromyces marxianus）对柑橘采后绿霉病菌的抑制效果，并观察其对采后柑橘果实多酚氧化酶（PPO）、过氧化物酶（POD）和苯丙氨酸解氨酶（PAL）活性的影响。结果表明：拮抗酵母菌的菌株悬浮液和培养原液对柑橘采后绿霉病菌均有较好的抑制作用，但菌株培养上清液和热杀死液对柑橘绿霉病菌的抑制效果较差；与对照相比，接种拮抗菌与病原菌能够诱导柑橘果实PPO、POD和PAL活性的升高；拮抗酵母菌对柑橘绿霉病菌的抑菌机制与营养和空间的竞争以及诱导抗病性相关酶有关。
Mostrar más [+] Menos [-]Inglés. The inhibitive activity of Kluyveromyces marxianus against citrus green mold caused by Penicillium digitatum Sacc. was investigated, as well as its effect on the activities of polyphenol oxi- dase (PPO), peroxidase (POD) and phenylalanine ammoniumlyase (PAL). The results showed that the treatments of washed cell suspension and living cell suspension have better inhibiting effect, as compared to the control treatment while the culture filtrate and autoclaved cell suspensions could exacerbate green mold decay. The application of K.marxianus could induce higher PPO, POD and PAL activity as compared to the control. It was suggested that the biocontrol mechanism might be competitive for nutrition and space, and contribute to the secretion of antagonistc substance.
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