Bioactivity of methanol extracts from Justicia procumbens L. of Chinese medicine against Ostrinia furnacalis Guenee | 中药爵床甲醇提取物对亚洲玉米螟的生物活性

Chino. 为评价爵床（Justicia procumbens L.）甲醇提取物对亚洲玉米螟取食、存活、生长发育的影响和产卵忌避效果，采用室内生测法测定了爵床提取物对亚洲玉米螟的触杀、胃毒、拒食、生长发育抑制和产卵忌避作用。结果表明，爵床甲醇提取物对亚洲玉米螟具有一定的触杀活性和较强的胃毒、拒食、生长发育抑制及产卵忌避活性。在触杀试验中，药后24h和72h爵床甲醇提取物32mg/mL处理亚洲玉米螟3龄幼虫死亡率分别为28.33%和43.33%。在选择性拒食试验中，药后24h和48h提取物对3龄幼虫的AFC50分别为3.10mg/mL和3.90mg/mL；在非选择性拒食试验中，药后24h和48h提取物对3龄幼虫的AFC50分别为4.44mg/mL和5.77mg/mL。在胃毒试验中，药后6d和7d提取物对3龄幼虫的LC50分别为5.09mg/mL和2.73mg/mL。爵床提取物对亚洲玉米螟幼虫的生长发育有明显抑制作用，表现在体质量、体质量增加量和相对生长率均显著降低，药后24h和48h IC50分别为0.84 mg/mL和0.53mg/mL。爵床提取物对亚洲玉米螟成虫产卵具有较强的忌避作用，药后24，48，72h提取物对亚洲玉米螟成虫的AOC50分别为6.73，9.71，12.29mg/mL。

Inglés. In order to ascertain the bioactivity of methanol extracts from Justicia procumbens, their effects on contacting toxicity, antifeeding, stomach poisoning, inhibition of growth and oviposition deterrence of Ostrinia furnacalis Guenee were bioassayed in the greenhouse. The results showed that the extracts from J. procumbens L. had the bioactivity of certain contacting toxicity, strong antifeeding, stomach poisoning, growth inhibition and oviposition-deterrent activities against O. furnacalis. The mortality of the extracts at the concentration of 32 mg/mL against 3rd instar larvae of O. furnacalis within 24 and 48 h after treatment reached 28.33% and 43.33%, respectively. The choice and non-choice AFC50 values within 24 and 48 h after treatment in the anti-feeding test were 3.10, 3.90 mg/mL and 4.44, 5.77 mg/mL, respectively. The LC50 values of extracts against 3rd instar larvae of O. furnacalis within 6 and 7 d after treatment in the stomach poisoning test were 5.09 and 2.73 mg/mL, respectively. The extracts from J. procumbens also imposed great influence on the growth of O. furnacalis Guenee larvae. The treated larvae showed big reduction in body weight, weight gain and relative growth rate compared with the control. The IC50 values within 24 and 48 h after treatment were 0.84 and 0.53 mg/mL, respectively. The extracts from J. procumbens had strong oviposition deterrence against O. furnacalis adult, the AOC50 values within 24, 48 and 72 h after treatment were 6.73, 9.71 and 12.29 mg/mL, respectively.