A study on the hypolipemic and nonenzymatic glycosylation activity in vitro of crude polysaccharide form nine plants | 9种植物粗多糖体外降脂及抑制非酶糖基化活性的研究

Chino. 对9种植物中的多糖进行提取，并对其体外降脂和抑制蛋白质非酶糖基化的作用进行了测定。分别将9种植物样品经粉碎、脱脂、脱色处理后，用超声辅助70%乙醇浸提得粗多糖。以葡萄糖为标准样品，用紫外分光光度法测其在510nm处吸光度值并绘制标准曲线，以同样的方法测定样品中多糖的含量。随后进行体外降脂实验，通过比较有无抑制剂（提取液）加入的情况下，脂肪酶的活性差异，所得各提取物的抑制率，比较降脂活性大小；再进行体外非酶糖基化实验，去1mL提取液（加氨基胍做阳性对照，加水做阴性对照）与1mL磷酸缓冲液混合，90℃水浴40min，然后与乙二醛和牛血清白蛋白混合，反应液于37℃孵育15d，用荧光法在发射波长440nm激发波长365nm处测定糖基化产物的自发荧光值，计算9种植物粗提多糖对体外非酶糖基化反应的抑制率。结果表明9种植物多糖粗提物对脂肪酶均有较强的抑制作用，以蛇接骨草的抑制活性最强。在体外非酶糖基化试验中，9种植物对非酶糖基化终产物的生成均有较强的抑制作用，以芦荟粗多糖非酶糖基化抑制活性最强。

Inglés. In this study, the hypolipemic and nonenzymatic glycosylation activity in vitro of rough polysaccharide from nine different plants were studied. The plant samples were smashed, degreased, bleached, then the rough polysaccharide was extracted with 70% ethanol and supersonic. The hypolipemic effect of the polysaccharide from nine plants was detected in vitro by measuring the activity of pancreatic lipase. In the nonenzymatic glycosylation experiment, the polysaccharide solution (1 mL) was mixed separately with PBS (1 mL), and water heated at 90℃ for 40 min to remove ethanol. Then the samples mixed with the reactive solution containing glyoxal and bovine serum albumin were incubated at 37℃ for 15 d, added with the aminoguanidine as the positive control while pure water as the negative control. The AGEs contents were assayed by the specific fluorescence with emission at 440 nm upon excitation at 365 nm to calculate the inhibitory effects of the polysaccharide from 9 different plants on nonenzymatic glycosylation. The results showed that the hypolipemic activities of rough polysaccharide from different plants on the lipase were different, and the activity of the Gynura procumbens (Lour.) Merr. was the best; and the nonenzymatic glycosylation activities of the polysaccharide from different plants were also varied, of all the polysaccharides, the effect of the Aloe was the best.