The Isolation and Culture Characterization of a Lipolytic Enzyme Producing Strain from Meju
2012
Yun, H.J., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea | Lee, Y.J., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea | Yeo, S.H., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea | Choi, H.S., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea | Park, H.Y., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea | Park, H.D., Kyungpook National University, Daegu, Republic of Korea | Baek, S.Y., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea
For screening of useful enzymes producing microorganisms from Meju, we isolated high lipase producing strains and their lipolytic enzyme activities were then tested. The lipolytic enzyme activities of isolated microorganisms were therefore tested on the Y124 strain. The gene sequence analysis of ITS from Y124 strain revealed Yarrowia lipolytica. Lipase production by the Y124 strain was studied in media containing various carbon sources. The Y124 strain drastically increased lipolytic enzyme activity in YPO media containing olive oil, as well as in YPDO media containing both olive oil and glucose. Maximal lipase production was achieved in YPD (yeast extract-peptone-D-glucose) media containing 0.7% olive oil when cultured at 30℃ for 8 hrs. The lipase produced from the Y124 strain showed the highest activity in p-NPO (p-nitrophenyl octanoate (C∧8)), amongst the various p-nitrophenyl esters.
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