Characterization of the Thermophilic Bacterium Geobacillus sp. Strain GWE1 Isolated from a Sterilization Oven
2013
Correa-Llanten, Daniela, Fundacion Biociencia, Santiago, Chile | Larrain-Linton, Juanita., Fundacion Biociencia, Santiago, Chile | Munoz, Patricio A., Fundacion Biociencia, Santiago, Chile | Boehmwald, Freddy., Fundacion Biociencia, Santiago, Chile | Blamey, Jenny M., Fundacion Biociencia, Santiago, Chile
A gram-positive, rod-shaped, spore-forming, motile thermophilic bacterium was isolated from a sterilization oven. The microorganism GWE1, formally named Geobacillus wiegelii identified as a member of the genus Geobacillus. GWE1 grew under aerobic conditions of between 60-80℃ (optimum 70℃), in a pH range of 3.0-8.0 (optimum pH⁷⁰ 5.8), and between 0 and 2 M NaCl (optimum 0.3 M). The membrane polar lipids were dominated by branched saturated fatty acids, which included as the major constituents; iso- 15:0 (13.3%), 16:1(ω7$) (12.8%), 16:0 (28.5%), iso-17:0 (13.5%) and anteiso-17:0 (12.3%). The DNA G+C content was 47.2 mol% (determined by HPLC). The 16S rRNA gene sequence of GWE1 showed a high similarity with Geobacillus caldoxylosilyticus (97%). However, the level of DNA-DNA relatedness was only 58%. These data suggest that GWE1 is probably a novel specie of the genus Geobacillus.
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