Development and characterization of nine microsatellite loci from the Korean hare (Lepus coreanus) and genetic diversity in South Korea
2012
Kim, S.I., Conservation Genome Resource Bank for Korean Wildlife, Seoul National University, Seoul, Republic of Korea | An, J.H., Conservation Genome Resource Bank for Korean Wildlife, Seoul National University, Seoul, Republic of Korea | Choi, S.K., Conservation Genome Resource Bank for Korean Wildlife, Seoul National University, Seoul, Republic of Korea | Lee, Y.S., Conservation Genome Resource Bank for Korean Wildlife, Seoul National University, Seoul, Republic of Korea | Park, H.C., Conservation Genome Resource Bank for Korean Wildlife, Seoul National University, Seoul, Republic of Korea | Kimura, Junpei, Seoul National University, Seoul, Republic of Korea | Kim, K.S., Seoul National University, Seoul, Republic of Korea | Min, M.S., Conservation Genome Resource Bank for Korean Wildlife, Seoul National University, Seoul, Republic of Korea | Lee, H., Conservation Genome Resource Bank for Korean Wildlife, Seoul National University, Seoul, Republic of Korea
The Korean hare, Lepus coreanus, is an important mammal in ecosystem food chains, and is distributed across the entire Korean peninsula and northeastern China. Polymorphic microsatellite loci were developed using the biotin-enrichment technique for use in population genetics studies. Five trinucleotide and four dinucleotide microsatellite loci were selected and tested on 22 Korean hare specimens collected from Gangwon Province and Gyeongsangbuk Province in South Korea. The number of alleles across the two sampling regions ranged from three to nine with a mean of 6.1. Mean observed and expected heterozygosities and polymorphic information content were 0.540, 0.627 and 0.579, respectively. Only one locus, Lc06, showed departure from Hardy-Weinberg equilibrium after applying the Bonferroni correction. Four microsatellites, Lc01, Lc03, Lc12, and Lc19, satisfied the criteria to serve as a core set of markers recommended for population genetics studies. These new microsatellite markers will be widely applicable to future genetic studies for management and conservation of the Korean hare and related species, including assessment of the genetic diversity and population structure of L. coreanus.
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