Preliminary study about sublingual administration of bacteria-expressed pandemic H1N1 influenza vaccine in miniature pigs
2014
Kim, H., Research Evaluation Team, Institute for Basic Science, Daejeon, Republic of Korea | Kim, J.K., College of Pharmacy, Korea University, Sejong, Republic of Korea | Song, H., Molecular Vaccinology Section, Laboratory Science Division, International Vaccine Institute, Seoul, Republic of Korea | Choi, J., Molecular Vaccinology Section, Laboratory Science Division, International Vaccine Institute, Seoul, Republic of Korea | Shim, B., Molecular Vaccinology Section, Laboratory Science Division, International Vaccine Institute, Seoul, Republic of Korea | Kang, B., Research Unit, Green Cross Veterinary Products, Yong-in, Republic of Korea | Moon, H., Research Unit, Green Cross Veterinary Products, Yong-in, Republic of Korea | Yeom, M., Viral Infectious Disease Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejon, Republic of Korea | Kim, S.H., Viral Infectious Disease Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejon, Republic of Korea | Song, D., Viral Infectious Disease Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejon, Republic of Korea | Song, M., Molecular Vaccinology Section, Laboratory Science Division, International Vaccine Institute, Seoul, Republic of Korea
Sublingual (SL) administration of influenza vaccine would be non-invasive and effective way to give human populations protective immunity against the virus, especially when pandemic influenza outbreaks. In this study, the efficacy of pandemic influenza virus-based subunit vaccines was tested after sublingual (SL) adjuvant administration in pigs. Eight specific pathogen-free Yucatan pigs were divided into 4 groups: nonvaccinated but challenged (A) and vaccinated and challenged (B, C, and D). The vaccinated groups were subdivided by vaccine type and inoculation route: SL subunit vaccine (hemagglutinin antigen 1 [HA1] + wild-type cholera toxin [wtCT], B); IM subunit vaccine (HA1 + aluminum hydroxide, C); and IM inactivated vaccine (+ aluminum hydroxide, D). The vaccines were administered twice at a 2-week interval. All pigs were challenged with pandemic influenza virus (A/swine/GCVP-KS01/2009 [H1N1]) and monitored for clinical signs, serology, viral shedding, and histopathology. After vaccination, hemagglutination inhibition titre was higher in group D (320) than in the other vaccinated groups (40-80) at the time of challenge. The mobility and feed intake were reduced in group C. Both viral shedding and histopathological lesions were reduced in groups B and D. Although this study has limitation due to the limited number of pigs (2 pigs per a group), the preliminary data in this study provided the protective potential of SL administration of bacteria-expressed pandemic H1N1 influenza vaccine in pigs. There should be additional animal studies about effective adjuvant system and vaccine types for the use of SL influenza vaccination.
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