Evaluation of anti-inflammatory and cytotoxic activities of stem and leaf extracts of a plant from the family Thymelaeaceae
2009
Ihsan Safwan K. | Mazura M P. | Fadzureena J. | Khoo M G H.
DT08/540 (stem) and DT08/541 (leaf) are the crude extracts of a plant from the family Thymelaeaceae. This plant is traditionally used in aroma-therapy. The present study sought to evaluate the efficacy and safety of these extracts. Methanol extracts of this plant were evaluated for anti-inflammatory activity based on hyaluronidase, lipoxygenase and xanthine oxidase inhibitory assays. The leaf extract (DT08/541) showed strong inhibition on hyaluronidase, lipoxygenase and xanthine oxidase with inhibition of 96.42 ± 4.08%, 81.34 ± 0.01%and 64. 13 ± 0.01% , respectively. However, the stem extract (DT08/541) exhibited low activity in all assays tested. Cytotoxic effect of the extracts was investigated in vitro using Sulforhodamine B (SRB) assay on normal kidney (Vero) and normal liver (WRL-68) cell lines. The results showed the median inhibitory concentration (IC50) of stem and leaf extracts in both cell lines ranged from 60 ug/ml to 375 ug/ml. Since both extracts showed a IC50 value above 50 ug/ml, it could be considered as non-cytotoxic. This study showed that the leaf extracts of this plant could be further explored for potential usage as remedies for inflammation treatment.
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