Effect of physicochemical parameters on lipase production by Penicillium fellutanum using canola seed oil cake as substrate
Amin, M. (University of Agriculture, Faisalabad (Pakistan). Dept. of Chemistry and Biochemistry) | Bhatti, H.N. (University of Agriculture, Faisalabad (Pakistan). Dept. of Chemistry and Biochemistry)
Microbial lipases show a unique behavior among other enzymes due to their ability to catalyze various reactions in aqueous and non-aqueous media. The present study was planned to explore new potential fungal strain for lipase production using agro-industrial wastes under solid state fermentation (SSF). Among different residues used as substrate such as rice bran, wheat bran, canola seed oil cake, sunflower hulls and peanut shells, the canola seed oil cake showed the highest lipase activity. Various production parameters like initial pH of production media, moisture content, incubation time, inoculum size, incubation temperature and substrate level were optimized to enhance extracellular lipase production. Effect of different carbon and nitrogen sources was also studied. A maximum lipase activity of 521 units/gram dry substrate (U/gds) was obtained after 48 h of reaction time in a media containing 10 g canola seed oil cake as a substrate supplemented with 2% olive oil as inducer and 50% moisture content at initial pH of 4.0 using 2 mL inoculum at 30 degree C. Additional carbon sources also affect significantly the fermentation process. Among different carbon sources glucose exhibited maximum potential to enhance the lipase production.
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