Research of variability in Prestice Black Pied pig using dinucleotide and tetranucleotide microsatellite markers
2013
Vrtkova, I., Mendel University, Brno (Czech Republic). Dept. of Morphology, Physiology and Animal Genetics | Stehlik, L., Mendel University, Brno (Czech Republic) | Putnova, L., Mendel University, Brno (Czech Republic)
The aim of this study was to use 10 dinucleotide and 12 tetranucleotide microsatellite markers (MS) to characterize the variability in 89 breeding boards of Prestice Black Pied Pig (PC). Mean number of alleles at dinucleotide versus tetranukleodtide MS was 7.40-7.55, observed heterozygosity 0.740-0.696 and PIC 0.691-0.682. We also evaluated diversity of PC and all other breeds kept in the Czech Republic (Large White - father line (LWF) and Large White - mother line (LWM), Landrace (L), Pietrain (P), Duroc (D), and Czech Meat (CM)) using 10 dinucleotide MS. Total of 658 breeding boars were analyzed. Mean number of alleles per locus in all breeds was 12.9, ranging between 9 to 18 alleles. The greatest PIC was 0.691 in PC and lowest 0.506 in D. The expected and observed heterozygosity was 0.760, 0.635 resp. across all loci. Only Pietrain breed showed a significant deficit in heterozygosity, which was 0.068. The analysis of population structure with using algorithm based on the Markov chain Monte Carlo method in Structure software confirms that there was little admixture between seven studied breeds, except Large White - father line and Large White - mother line.
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