Molecular systematic of Iranian Ixodidae on the basis of ITS2 analysis and comparison of its complete sequences between genera and Species.
2010
Abdigoudarzi, Mohammad | Ghoreyshi, Seyed Ali | Goudarzi, Gollam Hossein | Rivaz, Shalla
Hard ticks (Acari: Ixodidae) are important obligatory blood feeding external parasites of human and animals. There have been a lot of studies on taxonomy of Iranian ixodidae. There are problems in tick identification. Molecular taxonomic tools have been regarded and internal transcribed spacer2 has been selected to amplify the specific part of the genome of ticks. The purpose of the study was to use sequence variations of this marker to drive a molecular phylogeny. Ticks have been collected during favorable seasons in 2007. They have been diagnosed and DNA was extracted. After PCR amplification, fifty two specific bands have shown related to fifty two individuals after gel electrophoresis. Thirty clear bands have been purified and prepared for sequencing. The sequences have been aligned and released in PubMed and their accession numbers are listed. The levels of intraspecific and interspecific variations have been analyzed using suitable softwares. These variations yielded different phylogenetic arrangement of ITS2 sequences. The level of intraspecific variability of sequences within H. anatolicum anatolicum was high but for D. marginatus and R. turanicus appeared to be low. All D. marginatus sequences were at the same branch, H. dromedarii was most resembled to H. anatolicum anatolicum, and the sequence of R. bursa differed only by 16 fixed bp from the sequence of R. turanicus. These results indicate the advantage of ITS2-rDNA analysis in phylogeny of hard ticks and additional trials to collect more ticks from different places of Iran and to promote these data is in progress. Key words: Molecular taxonomic - Ticks- ITS2- Ixodidae - sequencing.
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