Anti-bacterial and Anti-inflammatory Effect of Melittin on Propionibacterium acnes-induced Inflammatory Skin Disease in vivo
2015
Lee, S.J., College of Medicine, Daegu, Republic of Korea | Kim, K.H., College of Medicine, Daegu, Republic of Korea | Lee, W.R., College of Medicine, Daegu, Republic of Korea | Kim, J.Y., College of Medicine, Daegu, Republic of Korea | An, H.J., College of Medicine, Daegu, Republic of Korea | Park, K.K., College of Medicine, Daegu, Republic of Korea
Recently, the therapeutic effect of bee venom on propionibacterium acnes (P. acnes)-induced inflammation has been known and the mechanism of melittin, one of the main component of bee venom, against acne arises as an interesting issue. In this study, we evaluated suppressive effect and a part of the mechanism of the P. acnes - induced inflammation in vivo. The agar diffusion method, the modified disk diffusion method, was used to assess growth inhibitory effects. Melittin (1, 10, 100 microgram) in vaseline was applied epicutaneously on the ear of mice resulting in P. acnesinduced inflammation. To investigate the anti-inflammatory response of melittin in P. acnes-induced inflammation, hematoxylin and eosin stain and immunohistochemical stain of F4/80 was done in the submitted ear specimen. Melittin inhibited the growth of P. acnes and this effect showed dose dependant and time dependant manner. Epicutaneous administration of melittin decreased inflammatory reaction associated with P. acnes and decreased number of macrophages, especially at the dose of 1microgram. From these results, we expect that melittin has strong antibacterial and antiinflammatory activities and has the potential as an alternative treatment to the antibiotic drug therapy of acne vulgaris.
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