Evaluation of genotoxic and cytotoxic effects of natamycin in mice bone marrow cells
2015
Rasgele, P.G. (Duzce Univ., Duzce (Turkey). Beekeeping Research Development and Application Center) | Kaymak, F. (Trakya Univ., Edirne (Turkey). Dept. of Biology)
Natamycin, q food preservative, is widely used in food industry against yeast and fungi. The potential genotoxicity of a commercial formulation of natamycin (Delvocid, containing 50% natamycin as the active ingredient) in mice bone marrow cells was investigated in vivo by chromosome aberrations (CA) and micronucleus assays (MA). Animals were intraperitoneally treated with 200, 400 and 800 mg/kg of natamycin for 6, 12 and 24 h in CA assay, for 24, 48 and 72 hours in MN assay. In the present study, natamycin did not increase chromosome aberrations. It significantly induced frequency of micronucleus at all concentrations for both 24 and 48 h in female mice, at the two highest (400 and 800 mg/kg) concentrations for both 24 and 48 h in male mice when compared with negative control. Mice bone marrow erythrocytes exposed to all concentrations of natamycin showed significant decreases in mitotic index for all treatment periods. In addition, natamycin reduced polychromatic erytrocyte/normochromatic erytrocyte ratio at all concentrations for 48h and at the highest (800 mg/kg) concentration for 24 and 72h in female mice; at all concentrations for 24 and 48h in male mice. The results of the present study show that a commercial formulation of natamycin was aneugenic and cytotoxic to mice bone marrow in vivo. For this reason it is necessary to be careful when using these chemicals in food as preservatives.
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