Effect of gomchwi (Ligularia fischeri) extract against high glucose- and H2O2-induced oxidative stress in PC12 cells
2016
Park, S.H., Gyeongsang National University, Jinju, Republic of Korea | Park, S.K., Gyeongsang National University, Jinju, Republic of Korea | Ha, J.S., Gyeongsang National University, Jinju, Republic of Korea | Lee, D.S., Gyeongsang National University, Jinju, Republic of Korea | Kang, J.Y., Gyeongsang National University, Jinju, Republic of Korea | Kim, J.M., Gyeongsang National University, Jinju, Republic of Korea | Lee, U., National Institute of Forest Science, Seoul, Republic of Korea | Heo, H.J., Gyeongsang National University, Jinju, Republic of Korea
Effects of the ethyl acetate fraction from gomchwi (Ligularia fischeri) extract against high glucose/H2O2- induced oxidative stress and in vitro neurodegeneration were investigated to confirm the physiological property of the extract. The ethyl acetate fraction of gomchwi extract showed the highest total phenolic contents than the other solvent fractions. An anti-hyperglycemic activity of the ethyl acetate fraction was evaluated using the alpha-glucosidase inhibitory assay, and the half maximal inhibitory concentration (IC∧50) value for alpha-glucosidase was found to be 727.64 μg/mL. In addition, the ethyl acetate fraction showed excellent 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) diammonium salt radical scavenging activity, and inhibition of malondialdehyde production. The ethyl acetate fraction also decreased intracellular reactive oxygen species, whereas neuronal cell viability against high glucose/H₂O₂-induced cytotoxicity was found to be increased. Finally, 3,5-dicaffeoylquinic acid as a main phenolic compound in the ethyl acetate fraction was analyzed by high-performance liquid chromatography. These results suggest that gomchwi might be a good natural source of functional materials to prevent diabetic neurodegeneration.
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