Characterization of Bacteriophages against Salmonella Gallinarum
2017
Kim, M., Kangwon National University, Chuncheon, Republic of Korea | Kwon, H.M., Kangwon National University, Chuncheon, Republic of Korea | Sung, H.W., Kangwon National University, Chuncheon, Republic of Korea
Bacteriophages are viruses that exclusively infect bacterial cells, and lytic bacteriophages can be used as a safe alternative to antibiotics for the prevention and treatment of animal diseases. In this study, we attempted to isolate and characterize bacteriophages for Salmonella enterica serovar Gallinarum (Salmonella Gallinarum), the causative agent of fowl typhoid in chickens. Ten bacteriophages were isolated from samples of sewage from seven poultry slaughterhouses. One of these isolate, designated as SGΦ-YS SP and classified in the family Myoviridae, produced plaques with seven Salmonella Gallinarum strains. However, no plaques were produced with any of the Salmonella enterica serovar Enteritidis strains tested, suggesting that this bacteriophage is Salmonella Gallinarum specific. To assess the lytic ability of SGΦ-YS SP against Salmonella Gallinarum, bacterial growth rates following inoculation of the bacteriophage were compared with the control. The SGΦ-YS SP treatment, with a multiplicity of infection of 10, reduced the growth of Salmonella Gallinarum by 2.21 log cfu/mL at 6 h, and 2.13 log cfu/mL at 9 h, suggesting that this bacteriophage isolate could be used for the prevention or treatment of Salmonella Gallinarum infection in chickens.
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