DNA Fingerprinting Analysis of Natural Monument Gingko Trees Using Microsatellite Markers
2017
Lee, J.W., National Institute of Forest Science, Suwon, Republic of Korea | Lee, M.W., National Institute of Forest Science, Suwon, Republic of Korea | Ahn, J.Y., National Institute of Forest Science, Suwon, Republic of Korea | Hong, K.N., National Institute of Forest Science, Suwon, Republic of Korea | Baek, S.H., National Institute of Forest Science, Suwon, Republic of Korea | Kim, S.C., National Institute of Forest Science, Suwon, Republic of Korea
This study describes DNA fingerprinting analysis of twenty-three natural monument individuals of Ginkgo biloba using eight microsatellite markers. The average number of observed alleles was 6.875, and the expected heterozygosity and the observed heterozygosity were 0.711 and 0.710, respectively. This results were similar to those of the previous studies on Ginkgo trees analyzed by same markers in China and Japan. PIC value and PD were calculated at 0.677 and 0.9999 respectively, indicating a high individual identification efficiency. In fact, all of the natural monument ginkgo trees and additionally analyzed thirteen general ginkgo tress were identified by genotype comparison. PI and PD calculated in three markers (Ging06, Gb60, Gb61) with the highest PIC values calculated in natural monument ginkgo trees were 8.045 x 10∨-5 and 99.99%, respectively. Thus, these three markers could be preferentially used in DNA fingerprinting for identifying ginkgo tree individuals. The results in this study will be useful for management of natural monument ginkgo trees, proliferation of their progeny and genetic identification of individuals selected in breeding process.
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