Plant Regeneration via Adventitious Shoot Formation in Platycodon grandiflorum (Jacq. A. DC.)
2017
Kim, J.Y., Nambu University, Gwangju, Republic of Korea | Na, H.S., Nambu University, Gwangju, Republic of Korea | Choi, P.S., Nambu University, Gwangju, Republic of Korea
To investigate optimal conditions for plant regeneration in Platycodon grandiflorum (Jacq. A. DC.). Both leaf and hypocotyl explants were cultured on Murashige and Skoog's (MS) medium supplemented with combinations of 0.1, 0.5, 1.0, or 2.0 mg/L cytokinins (BA and kinetin) and 1.0 mg/L 2,4-D for 6 weeks, respectively. According to the type of explant, the total shoot organogenesis (56.38%) in leaf explants was higher than in hypocotyls (28.20%). In comparison with kinetin and BA for the plant regeneration, the frequency (70.38%) of leaf explants was higher in combination with kinetin and 2,4-D than of BA with 2,4-D (42.38%), whereas the frequency (35.56%) of hypocotyls explants was higher in BA combination than kinetin combination (20.83%). Thehighest frequency (94.20%) was observed from the cultures of leaf explants on the MS medium supplemented with 1.0 mg/L kinetin and 1.0 mg/L 2,4-D. Upon transfer onto 1/2 MS basal medium containing 3% sucrose, shoots developed into plantlets with roots, and were well grown in soil in the greenhouse. These results lead us to speculate that the optimization of culture conditions was responsible for the mass propagation from in vitro cultures of Platycodon grandiflorum (Jacq. A. DC.).
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