Flavonoid Glycosides from the Flowers of Pulsatilla koreana Nakai
2016
Seo, K.H., Kyung Hee University, Yongin, Republic of Korea | Jung, J.W., Kyung Hee University, Yongin, Republic of Korea | Thi, N.N., Kyung Hee University, Yongin, Republic of Korea | Lee, Y.H., Kyung Hee University, Yongin, Republic of Korea | Baek, N.I., Kyung Hee University, Yongin, Republic of Korea
Extraction and fractionation of Pulsatilla koreana flowers followed by, repeated open column chromatography for EtOAc and n-BuOH fractions yielded four flavonoid glycosides, namely, astragalin (1), tiliroside (2), buddlenoide A (3), and apigenin-7-O-(3"-E-p-coumaroyl)-glucopyranoside (4). The chemical structures of these flavonoid glycosides were elucidated on the basis of various spectroscopic methods including electronic ionization mass spectrometry (EI-MS), 1D NMR (¹H, ¹³C, DEPT), 2D NMR (gCOSY, gHSQC, gHMBC), and infrared (IR) spectrometry. This study represents the first report of the isolation of the flavonoid glycosides from the flowers of P. koreana.
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