HPLC - UV analysis of sample preparation influence on flavonoid yield from Cirsium japonicum var. maackii
2017
Joyce P. Rodriguez, Chung-Ang University, Anseong, Republic of Korea | Lee, J., Chung-Ang University, Anseong, Republic of Korea | Park, J.Y., Gachon University, Seongnam, Republic of Korea | Kang, K.S., Gachon University, Seongnam, Republic of Korea | Hahm, D.H., Kyung Hee University, Seoul, Republic of Korea | Lee, S.C., Imsil Cheese and Food Research Institute, Imsil, Republic of Korea | Lee, S., Chung-Ang University, Anseong, Republic of Korea
This study was conducted to optimize the extraction conditions of flavonoids from Cirsium japonicum var. maackii (ICF-1). The effects of sample material ratio, solvent concentration, extraction time, solid-to-solvent ratio, and number of extractions on flavonoid extraction efficiency were analyzed. Three flavonoids were specifically investigated: cirsimarin (1), hispidulin (2), and cirsimaritin (3). In single-factor experiments, each variable had a significant effect on the determination of content of compounds 1 - 3. The optimal conditions for extraction were found to be: mass, 15 g; ratio of spring and fall leaves, 4:1; extraction solvent, 70% ethanol; extraction time, 4 h; solid-to-solvent ratio, 1:20; and number of extractions, 1. The results of the study were used to maximize the potential of ICF-1 samples and optimize the efficiency of the extraction process.
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