Evaluation of moringa (Moringa oleifera) leaves extract on the development of citrus nematode (Tylenchulus semipenetrans)
2015
Naeem, I.
The efficacy of different concentrations of moringa leaves extract (methanolic extracts, ethanolic extracts, water extracts) was evaluated. In J2 mortality experiment 5ml of different concentrations of moringa leaves extract was placed in plastic plate. Then add 1ml nematode suspension carrying 25 freshly hatched J2 with the help of pipette. Maximum J2 mortality (100%) was observed in methanolic extracts concentration. Invasion and development of citrus nematode were evaluated on citrus crop. Data was recorded after 1, 6 and 12 weeks to observe the penetration and development stages including vermiform, Swollen, Sausage stage, Immature females and mature females of T. semipenetrans. Maximum reduction of T. semipenetrans was observed when soil was treated with methanolic extract of moringa leaves. Most effective concentration of moringa leaves extract determined by above experiments was used to assess its effect as protective and curative. The freshly hatched 1000 J2 per plant were used as inoculum. There were 3 treatments and 5 replications. Moringa leaves extract was applied before inoculation as protective and after inoculation as curative effect. The plant growth parameters including plant height, root length and number of leaves and nematodes parameters including number of females/root system, no of juveniles/root system and no of juveniles/100 ml3 soil were recorded after 60 days of growth. Citrus nematode population was reduced when methanolic extracts of moringa leaves was applied as protective application.
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