Evaluation of biotoxicant profiles in Millardia meltada

Present study was conducted to report the effect of 4000 mg/kg ethanolic leaf extract of Datura stramonium, Eucalyptus camaldulensis and a mixture of both with (1: 1 ratio) for 2 weeks. Normal feed treated T0 was maintained in parallel for each treated three experimental groups T1, T2, T3 were given the ethanolic leaf extract of Datura stramonium, Eucalyptus camaldulensis and both respectively irrespective of gender in the form of bait. Blood samples from 20 Millardia meltada rats irrespective of gender [Datura stramonium, Eucalyptus camaldulensis and mixture of Datura stramonium and Eucalyptus camaldulensis treated (N = 15) and normal feed treated rats (N= 5)] were collected and various hematological and serum biochemical parameters were determined. The maximum feed consumption was 128.72 plus minus 2.31, 132.2 plus minus 2.58 respectively under control rats and maximum water intake in 1st and 2nd week was 67 plus minus 2.73, 77.58 plus minus 1.66 respectively under Datura stramonium treated rats. A decrease in weight was observed in treated rats. The maximum decrease in weight was 38.8 plus minus 4.71 from 51.2 plus minus 544 in Eucalyptus camaldulensis treated rats. Fur color was faint out and fur texture became hard and coarse in treated rats. The MCV (64.74 plus minus 0.43), MCH (45.56 plus minus 0.43), MCHC (68.74 plus minus 0.75), PLT (551.2 plus minus 1.30), cholesterol (110.4 plus minus 1.14), triglyceride (239 plus minus 1.58), billirubin (0.88 plus minus 0.13), SGPT (59 plus minus 1.58), SGOT (231.6 plus minus 1.14) was significantly higher in Datura stramonium treated rats than control, Eucalyptus camaldulensis and in a mixture of both plants extract treated rats. RBC (6.01 plus minus 0.08), HCT (33.2 plus minus 1.30), HGB (11.5 plus minus 0.40), LDL (42.2 plus minus 1.48) was significantly higher in control rats than Dalura stramonium, Eucalyptus camaldulensis, and a mixture of both plants extract treated rats. WBC (4.04 plus minus 0.29), NED (36.8 plus minus 1.48), total protein (7.9 plus minus 0.84) was significantly higher in Eucalyptus camaldulensis treated rats than control, Datura stramonium and mixture of both plants extract treated rats. LYM (82 plus minus 1.58), HDL (68.34 plus minus 1.21) was significantly higher in mixture of both plants extract treated rats than control, Datura stramonium and Eucalyptus camaldulensis treated rats. Histopathology, extracts caused liver and kidney damages in Millardia meltada. The alterations on some hematological, biochemical parameters, liver and kidney damage were an indication that the 4000 mg/kg ethanolic leaf extracts possess selectively toxicity.