Determination of tocopherol and carotenoid contents in ST muscle of suckling lambs using fresh or lyophilised muscle
2016
Blanco, M | Molino Gahete, F | García-Durillo, M | Ripoll García, G | Lobón Ascaso, S | Sanz Pascua, A | Joy Torrens, M
The aim of the study was to compare tocopherol and carotenoids contents in lyophilised and fresh meat to reduce the time and chemical reactives used in the analytical procedure. Twenty ewe-lamb pairs grazed on pastures and 19 pairs were raised indoors with ewes receiving pasture hay. After slaughter (live-weight: 11 plus minus 0.8 kg), both semitendinosus muscles were excised to determine carotenoids and tocopherol contents in lyophilised or fresh meat. The contents of Alpha-tocopherol and lutein determined using lyophilised meat were 2.8-fold and 21.3-fold greater than using fresh meat. The content of Beta-carotene was undetectable whereas Gamma-tocopherol content was not detected in lyophilised meat and near 0 in fresh meat. Regarding the effect of the ewe’s diet, Alpha-tocopherol content was greater in grazing than in hay treatment, regardless of the method of extraction. Lutein content was greater in grazing than in hay treatment lambs in lyophilised meat but did not differ in fresh meat. The con- tent of Gamma-tocopherol content was greater in grazing than in hay treatment lambs in fresh meat. Consequently, it would be more appropriate to determine Alpha-tocopherol and lutein contents in lyophilised meat, although Gamma-tocopherol content was not detectable
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