Low risk of pollen-mediated gene fow in transgenic plants under greenhouse conditions
2018
Kim, D.S., Chung-Ang University, Seoul, Republic of Korea | Sonng, I., Chung-Ang University, Seoul, Republic of Korea | Ko, K., Chung-Ang University, Seoul, Republic of Korea
There is concern about the potential for transgene contamination in wild type (WT) plants via pollen-mediated gene fow from transgenic plants. In this study, we investigate pollen-mediated gene fow using tobacco transgenic lines carrying recombinant protein GA733-FcK, which is a putative candidate for producing colorectal cancer vaccine. Transgenic and WT plants were grown in greenhouse, with WT plants placed 0.3, 1, 5, 10, and 20 m away from transgenic plants. Seeds were harvested from randomly selected WT plants and sown on germination media supplemented with or without kanamycin. At 30 days after sowing, none of the WT seedlings produced true leaves and roots when grown on selective media. Polymerase chain reaction analysis revealed that GA733-FcK and nptII genes were expressed in shoots of transgenic plants but not of WT plants. Expression of GA733-FcK and nptII proteins was also abolished in WT leaves when compared to that of transgenic plants. Our fndings suggested that there is low risk of pollen-mediated gene fow from transgenic plants expressing GA733-FcK when grown in greenhouse conditions.
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