Morphological and phylogenetic study of Philippine native pigs (Sus scrofa L.) of Quezon and Marinduque [Philippines] based on morphometric methods and diversity of microsatellite markers
2015
Logrobio, D.J.C.
High salt method developed by the School of Biological Sciences at the University of Liverpool had comparable quality and quantity of DNA with SolGent sup TM Genomic DNA Prep Kit (Solution Type); therefore it can be used as an alternative to the kit for the extraction of genomic DNA from ear tissues of Philippine native pigs. This study also determined the extent of genetic diversity of Quezon and Marinduque [Philippines] native pigs based morphological and nine microsatellite markers recommended by FAO (2004). Marinduque native pigs with a mean effective number of alleles (mean EA), mean observed heterozygosity (mean H sub O), mean expected heterozygosity (mean H sub E) and mean polymorphic information content (mean PIC) of 2.80 +- 0.75, 0.52 +- 0.12, 0.62 +- 0.12, and 0.56 +- 0.12, respectively possessed a slightly higher genetic diversity than Quezon native pigs (mean EA = 2.63 +- 0.67, mean H sub O = 0.50 ± 0.18, mean H sub E = 0.60 ± 0.14, and mean PIC = 0.55 +- 0.12). The F sub IS¬ = 0.1644 in both populations differed from each other; however, low genetic differentiation was observed (F sub ST = 0.0256; Nei's Standard genetic distance = 0.0652). The results may imply the need for further genetic improvement, conservation of these pigs and its development as Philippine native signature pig breeds.
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