Determinación de los requerimientos de vitamina K en larvas de corvina (Argyrosomus regius)
2016
Ramírez bolaños, S.
Despite the great interest of Argyrosomys regius in the diversification of the European aquaculture, as it is shown with the existence of a European framework for the development of the culture of this specie like the European Project DIVERSIFY, there are only a few studies about the meagre larvae culture and its nutritional requirements, and specially vitamin K requirements. Therefore, the main objective of this study was to determine the vitamin K requirements for the optimum meagre larvae growth and development. For that, five experimental diets with different vitamin K levels, from no addition of vitamin k to 14.30 mg per kg, were tested on meagre larvae since 18 days post hatching during 15 days feeding. The implications of the different vitamin K levels on larvae growth and survival rate were analysed in each experimental group. Thus, the larvae fed 5.90 mg per kg vitamin K in diet showed the highest survival rate and growth, whereas the poorest survival rate were observed in the larvae fed the diet without addition of vitamin K and with the diet containing 14.30 mg per kg of vitamin K. The histological analysis showed a better lipid utilization related to the amount of vitamin K in the diets. Regarding bone anomalies, a decrease in the anomalies incidence was observed when high levels of vitamin K in diets were present. By last, the vitamin K protein dependent gene expression analysis showed an overexpression of MGP related to the vitamin K amount in the experimental diets. The results obtained indicated that the vitamin K level for the optimum meagre larvae culture is around 5.90 mg per kg.
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