The Activity of Antioxidant Defence Enzymes (Glutathione Peroxidase , Glutathione Reductase and Phase II Biotransformation Enzyme Glutathione-S-Transferase) in Some Tissues of Stone Crayfish (Austropotamobius torrentium Shrank) from Krajkovacka River
2016
Milošević, Slaviša M. (Faculty of Science and Mathematics, Kosovska Mitrovica (Serbia)) | Živić, Nebojša V. (Faculty of Science and Mathematics, Kosovska Mitrovica (Serbia)) | Milosavljević, Maja V. (Faculty of Science and Mathematics, Kosovska Mitrovica (Serbia)) | Jakšić, Tatjana R. (Faculty of Science and Mathematics, Kosovska Mitrovica (Serbia))
The activity of the antioxidant defense enzymes glutathione peroxidase (GSH-Px, EC 1.11.1.9), glutathione reductase (GR, EC 1.6.4.2) and the phase II biotransformation enzyme glutathione-S-transferase (GST, EC 2.5.1.18) in some tissues of stone crayfish (Austropotamobius torrentium) were studied. In our study we investigated the activities of: glutathione peroxidase (GSH-Px), glutathione reductase (GR) and phase II biotransformation enzyme glutathione-S-transferase (GST) in the hepatopancreas, the gills and muscle of Stone crayfish (Austropotamobius torrentium). All enzyme activities were measured spectrophotometrically and presented as specific (U/mg proteins) and total values (U/mg wet mass). The results show strong tissue specificity of investigated enzymes. Specific and total GSH-Ph activity in abdominal muscle was lower than in hepatopancreas and gills. At the same time total GSH-Ph activity in gills was significantly lower in respect to hepatopancreas. The specific and total activity of GR was significantly higher in gills than in muscle and hepatopancreas, as well as in muscle in respect to the hepatopancreas. The specific and total activity of GST was significantly increased in hepatopancreas than in gills and muscle. At the same time, the activity of GST was higher in gills than in muscle. In the presented study our results show that both specific and total glutathione redox cycle enzyme activities GSH-Ph, GR and phase II biotransformation enzyme GST show a strong tissue specificities and present a good environmental markers.
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